منابع مشابه
Endothelial fenestral diaphragms: a quick-freeze, deep-etch study
The route by which water, solutes, and macromolecules traverse the endothelial cell has long been a subject of study for both physiologists and cell biologists. Recent physiologic studies describe a slit-shaped pore (5.1-5.7-nm wide) as the communicating channel, although no channel of such dimensions has been visible in electron microscopic preparations. That this channel should be found withi...
متن کاملThe extinction of the woolly mammoth: was it a quick freeze?
Apart from formerly glaciated areas, woolly mammoth remains are abundant in the surficial sediments of the mid and high latitudes of the Northern Hemisphere, including western Europe, northern and eastern Asia, Alaska and the Yukon. There are probably millions of mammoths buried in the permafrost of Siberia alone. The mammoths are found with a wide variety of other mammals, large and small, man...
متن کاملAge-related changes in human macular Bruch's membrane as seen by quick-freeze/deep-etch.
Lipid-containing inclusions have been observed in human Bruch's membrane (BrM) and are postulated to be associated with age-related maculopathy (ARM), a major cause of legal blindness in developed countries. The dehydration associated with specimen preparation for thin-section transmission electron microscopy causes loss of these inclusions. Better preservation of the ultrastructure of the incl...
متن کاملTandem high-pressure freezing and quick freeze substitution of plant tissues for transmission electron microscopy.
Since the 1940s transmission electron microscopy (TEM) has been providing biologists with ultra-high resolution images of biological materials. Yet, because of laborious and time-consuming protocols that also demand experience in preparation of artifact-free samples, TEM is not considered a user-friendly technique. Traditional sample preparation for TEM used chemical fixatives to preserve cellu...
متن کاملThe Chlamydomonas Cell Wall Glycoproteins Analyzed by the Technique and Its Constituent Quick-Freeze, Deep-Etch
Using the quick-freeze, deep-etch technique, we have analyzed the structure of the intact cell wall of Chlamydomonas reinhardi, and have visualized its component glycoproteins after mechanical shearing and after depolymerization induced by perchlorate or by the wall-disrupting agent, autolysin. The intact wall has previously been shown in a thin-section study (Roberts, K., M. Gurney-Smith, and ...
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ژورنال
عنوان ژورنال: Science
سال: 2013
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.339.6122.885-a